Current Issue : July-September Volume : 2018 Issue Number : 3 Articles : 4 Articles
In the present study simple, precise, rapid, sensitive, accurate and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated for sertraline hydrochloride. The chromatographic separation was carried out by using reverse phase stainless steel column of (4.5 mm × 25 cm) Intersil® C18 column with 5 μm (particles packing) held at 25°C. The mobile phase consisted of acetonitrile: sodium phosphate buffer of pH 4.7 (88:12 v/v), with a flow rate of 1.0 ml/ min, UV detection at 271 nm and injection volume 20 μl was maintained. The retention time was found to be 3.628 min. The different analytical performance parameters such as linearity, accuracy, precision and sensitivity (limit of detection and limit of quantitation) were determined according to International Conference on Harmonization (ICH) guidelines. The linearity was observed in the range of 50-300 μg/ml with correlation coefficient, r = 0.996....
Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated using solid phase extraction (SPE) technique for the determination of azilsartan and amlodipine in human plasma; chromatographic separation of the analyte was achieved at 7.61 min and 18.49 min for amlodipine and azilsartan respectively. HPLC column C 18, 150 X 4.6, 5µ, flow rate was 1.2 ml/ min, column oven temperature 25°C, injection volume 50 µl and mobile phase buffer was 50 mM potassium dihydrogen ortho phosphate buffer pH 7.00 and the detection was carried out at 240 nm. Calibration curve was linear (r2>0.9998) in the range of 10-60 ng/ml and 25-150 ng/ml for amlodipine and azilsartan respectively. No any endogenous constituents were found to interfere at retention time of the analyte. This new RP-HPLC method was successfully validated and may be applied to conduct bioavailability and bioequivalence studies....
The objective of the work was to establish a new, rapid and sensitive RP-HPLC method for determination of two commonly prescribed drug; miconazole and ornidazole. The RP-HPLC separation of the analytes was performed on a hypersil BDS C18 (250 mm x 4.6 mm, 5 µm) column, using isocratic elution of the mobile phase composed of potassium dihydrogen phosphate buffer (pH 3.5) and methanol (70:30 % v/v) with a flow rate of 1.0 ml/min. The multiple wavelength UV detector was set at a 230 nm for measurement of all compound. Quantification based on measuring the peak areas. The Two compound were resolved with retention times 4.227 for ornidazole and 5.413 for miconazole respectively. The calibration curves were linear in the range of 25-75 µg/ml and 5-15 µg/ml for ornidazole and miconazole respectively, all of them with coefficient of determination above 0.999. The methodology recoveries were higher than 99%. The limits of detection (LODs) were 1.140 µg/ml and 0.368 µg/ml for ornidazole and miconazole respectively. The intra-day coefficient of variation % RSD=0.213-0.667 and 0.254-1.40 for ornidazole and miconazole and inter-day coefficient of variation % RSD=0.717-1.408 and 1.24-1.74 for ornidazole and miconazole. The method was accurate, sensitive and simple for quality control as well as for stability indicating purpose....
The aim of this present work was to develop and validate a simple, rapid, accurate RP-HPLC method for the estimation of dolutegravir sodium in bulk. Mobile phase consisted of acetonitrile and HPLC water in the ratio of 1:1% v/v and was run through a column of Agilent TC-C18 (2), 5 µm, 4.5×250 nm at a flow rate of 1.5 ml/min and the eluents were monitored at 250 nm. The percent recoveries ranged between 95-105 % and RSD < 2%. Linearity was observed in the concentration range between 20 to 100% with correlation coefficient >0.995¬¬¬¬. The percent of relative standard deviation of six replicate measurements was found to be 0.972 which indicates that the method was precise. The proposed RP-HPLC method was validated according to ICH guidelines and was successfully employed for routine quality control analysis in bulk....
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